Overview
Advantages of ToolGen's Genome Edited Cell Line Production Service
Customized Production
- Optimized sgRNA selection and cell line engineering through pre-consultation to meet the client’s specific objectives.
- Provides tailored strategies for cell lines that are difficult to edit, ensuring successful genome modification.
High-Efficiency Editing
- Mixed Cell Pool Service: Provides pools with up to 60% In/Del efficiency.
- Single Cell Clone Service: Enables selection of clones with 100% In/Del efficiency.
- Utilizes highly specific sgRNA and transfection with Cas9 protein (RNP) or mRNA to minimize off-target effects.
Various Applications
- Gene function research
- Customized disease model cell production
- Cell and gene therapy research and development
툴젠의 IVT(In Vitro Transcription) 서비스의 강점
- 고객이 제공한 DNA template 기반으로 IVT 진행
- 연구 목적에 따라 안정성이 높은 modified mRNA (5moU) 옵션 선택 가능
- 원하는 scale에 맞춰 생산 가능
Workflow
Pre-Selected sgRNA
| Service | Item Descriptions | Period |
|---|---|---|
| Knock-Out Cell Line Service | Mixed Cell pool | 3-5 weeks |
| Single Cell Clone | 6-8 weeks | |
| Knock-In Cell Line Service | Mixed Cell pool | 8-10 weeks |
| Single Cell Clone | 11-13 weeks |
Novel sgRNA Screening
| Service | Item Descriptions | Period |
|---|---|---|
| Knock-Out Cell Line Service | Mixed Cell pool | 5-7 weeks |
| Single Cell Clone | 8-10 weeks | |
| Knock-In Cell Line Service | Mixed Cell pool | 10-12 weeks |
| Single Cell Clone | 13-15 weeks |
Optional QC Service
| Assay | Methods |
|---|---|
| Expression analysis | RT-qPCR, Western blot, Flow cytometry |
| Chromosome analysis | Karyotyping (G-banding) |
| Off-target analysis | NGS-based analysis |
In Vitro Transcription (IVT) RNA Synthesis Services
Cas9 & Customized mRNA IVT Synthesis (with Capping and Poly(A) Tailing)
| Service | Item Descriptions | Product yield | Period |
|---|---|---|---|
| Unmodified mRNA IVT | Requires DNA template vector containing T7 promoter | ≥30 µg | 1-2 weeks |
| Modified mRNA IVT (5-methoxyuridine) |
Guide RNA (gRNA) IVT Synthesis (PCR-based template)
| Service | Item Descriptions | Product yield | Period |
|---|---|---|---|
| gRNA IVT | PCR-based DNA template required; customer provides gRNA sequence | ≥30 µg | 1–2 weeks |
gRNA Design & Screening
| Service | Item Descriptions |
|---|---|
| gRNA selection | Bioinformatics-based gRNA design and selection (based on target sequence) |
Validated through National R&D Projects
툴젠은 국가 연구개발 과제를 통해 검증된 기술력을 확보하였습니다.
알키미스트 프로젝트
산업통상자원부 지원
면역거부반응이 없는 소프트 임플란트 개발
유전체 편집 기술 개발 과제
과학기술정보통신부 지원
차세대 지질나노입자 기반 생체 내 유전체 편집기술 개발